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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Nature. 2017 Jun 28;547(7661):114–117. doi: 10.1038/nature22990

Figure 1. Drosha mediates miRNA-independent antiviral activity.

Figure 1

a, Northern blot (NB) of RNA from NoDice and RNaseIII−/− cells reconstituted with indicated plasmids. Blots probed for miR-93 and U6. b, Western blot (WB) of whole cell extract from NoDice and RNaseIII−/− cells infected with SINV (MOI=0.01) at 4, 8, and 12 hpi. Blot probed for SINV Capsid (SIN-C) and pan-Actin (Actin). c–f, WBs of Ross River virus (MOI=0.1) (c), Langat virus (MOI=0.1) (d), Influenza virus (MOI=1) and (e), GFP-encoding Sendai virus (MOI=1) (f). Protein levels were assessed at 24 hpi using virus-specific or GFP antibodies as indicated. g, RNA-Seq correlation analyses of NoDice and RNaseIII−/− cells at baseline. h, As described in g, except cells were treated with dsRNA for 8 hrs. Graphs in g and h depict data from biological replicates.