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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Nature. 2017 Jun 28;547(7661):114–117. doi: 10.1038/nature22990

Extended Data Figure 6. Localization of and miRNA production from cytoplasmic viruses in diverse eukaryotes.

Extended Data Figure 6

a, Zebrafish embryos were inoculated with SINV for 24 hrs and then analyzed by fluorescence in situ hybridization (FISH) using a probe complementary to the capsid region of the genome. b, qPCR analysis of zebrafish embryos treated with indicated morpholinos for 2 days (n=4). c, Arabidopsis thaliana protoplasts were mock or TCV-infected for 40 hrs and then analyzed by FISH using a Cy3-labelled probe complementary to bases 1210–1259 of the TCV genome. d, Quantification of mature miR-124 production from recombinant TCV was performed using the TaqMan miRNA assay on RNA from Fig. 4b. All samples were normalized to endogenous snoR66. Quantifications of each sample were performed in triplicate and error bars denote standard deviation from two biological replicates.