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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Nature. 2017 Jun 28;547(7661):114–117. doi: 10.1038/nature22990

Figure 2. The RNA-binding domain of Drosha is essential for virus inhibition.

Figure 2

a, Core domains and variants of Drosha including: Proline-rich, P-rich; arginine serine-rich, RS-rich; Conserved Central Domain, CED; RNAseIII domain (RIIID) and double-stranded RNA binding domain (dsRBD). Black boxes indicate point mutations (mut) b, NB and WB of RNaseIII−/− cells transfected with the indicated Drosha variants and cytoplasmic miR-124. WB depicts Drosha, Dicer, and pan-Actin. c, WB and d, titers from RNaseIII−/− cells expressing the indicated transcripts and SINV RNA 24 hpt. Graph denotes average titers obtained with error bars generated using standard deviation from three independent experiments. ‘ns’ denotes not significant, all other conditions had a p-value of <0.05. e, WB as described in c with Drosha RBmut. f, WB of input and Flag-immunoprecipitated (IP) fractions derived from 293T cells expressing indicated transcripts and Flag-DGCR8.