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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Nature. 2017 Jun 28;547(7661):114–117. doi: 10.1038/nature22990

Figure 4. RNase III inhibition of virus replication is highly conserved.

Figure 4

a, NB of RNA from zebrafish embryos treated with indicated morpholinos, and inoculated 2 days later with wild type SINV (WT) or a strain encoding an artificial miRNA (amiRNA). Blot depicts amiRNA and U6 at 40hrs post infection b, NB of RNA from Arabidopsis thaliana protoplasts treated with a Turnip crinkle virus (TCV) containing a scrambled sequence (Scbl) or miR-124 (124). c and d, WB from RNaseIII−/− cells, co-transfected with the indicated plasmids and either in vitro transcribed SINV gRNA or Langat virus rescue plasmid. e and f, Small RNA-Seq of DL1 cells treated with indicated dsRNA for 3 days and subsequently infected with SINV (MOI=1) (e) or DCV (MOI=7) (f). Graphs depict the number of reads mapping to indicated positions along the viral genomes.