Extended Data Figure 3. Characterization of Drosha-2A cells.

a, Immunofluorescence of human fibroblasts stably expressing GFP-tagged Drosha-WT or Drosha-2A (S300A/S302A). b, The indicated Flag-tagged proteins were immunoprecipitated from whole cell extracts (WCE) and incubated at 37 °C with in vitro transcribed genome of SIN124. Production of pre-miR-124 was determined by small RNA northern blot. c, Indicated cell types were infected with SINV at an MOI of 0.001 and viral titers were determined at 16 hpi. Shown is average and standard deviation of three independent experiments, with p<0.05 as determined using a two-tailed student’s t-test.