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(A) Histology of malignant melanoma metastasis (biopsy from Fig. 1A) with hematoxylin and eosin staining; magnification, ×200. (B) Immunohistochemical analysis (hematoxylin counterstained) confirmed smooth muscle actin-positive myofibroblasts (brown) of tumor stroma between tumor lobules and also (C) transforming growth factor β1intensely positive melanoma cells in the lobules of melanoma metastasis. (D) Negative control.
