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. 2018 Feb 8;41(5):2755–2763. doi: 10.3892/ijmm.2018.3476

Figure 4.

Figure 4

LMO3 knockdown suppresses the phosphorylation of Akt, mTOR and GSK3β. (A) Western blot analysis of phospho-Akt and total-Akt in LMO3-knockdown and control MGC-803 cells and (B) quantified phospho-Akt/total-Akt ratios. (C) Western blot analysis of phospho-mTOR and total-mTOR in LMO3-knockdown and control MGC-803 cells and (D) quantified phospho-mTOR/total-mTOR ratios. (E) Western blot analysis of phospho-GSK3β and total-GSK3β in LMO3-knockdown and control MGC-803 cells and (F) quantified phospho-GSK3β/total-GSK3β ratios. **P<0.01. LMO3, LIM domain only 3; NC, negative control; siRNA, small interfering RNA; mTOR, mammalian target of rapamycin; GSK, glycogen synthase kinase.