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. 2018 Feb 5;41(5):2704–2714. doi: 10.3892/ijmm.2018.3457

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Copyright: © Ling et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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(A) The knockdown efficiency of the lncRNA ENSRNOG00000037522 was examined with reverse transcription-quantitative polymerase chain reaction, and a significant abrogation of lncRNA ENSRNOG00000037522 was observed following transfection with siRNA1 or siRNA2. (B) Western blot analysis was conducted to determine the α-SMA, vimentin, nephrin and POXLD1 expression levels in lncRNA ENSRNOG00000037522 knockdown and control podocytes. (C) Protein levels were quantified by ImageJ software and are presented in the histogram. Podocytes transfected with NC siRNA served as the controls. All experiments were performed in triplicate, and the results are presented as the mean ± standard deviation. ^*P<0.05 vs. NC group. lncRNA, long noncoding RNA; NC, negative control; siRNA, small interfering RNA.