Figure 5.

Inhibitory effects of Ir-3 on PLCγ2, PKC, and Akt activation in platelets. Washed platelets were preincubated with the solvent control (0.1% DMSO) or Ir-3 (10 and 20 µM) and subsequently treated with 1 µg/ml collagen or 150 nM PDBu to induce (A) PLCγ2 and (B) PKC activation (pleckstrin phosphorylation) or (C) platelet aggregation and (D) Akt activation. The profiles in section C are representative of four independent experiments. Data are presented as the mean ± standard error of the mean (n=4). ^***P<0.001 vs. resting control and ^###P<0.001 vs. the DMSO-treated group. PLCγ2, phospholipase Cγ2; PKC, protein kinase C; Akt, protein kinase B.