Figure 6.

Effects of Ir-3 on p38 MAPK, ERKs, and JNK1 phosphorylation in collagen-activated platelets. Washed platelets (1.2×10⁹ cells/ml) were pretreated with the solvent control (0.1% DMSO) or Ir-3 (10 and 20 µM) and then treated with 1 µg/ml collagen to stimulate platelet activation. Platelets were collected, and their subcellular extracts were analyzed to determine the levels of (A) p38 MAPK (n=3) (B) ERKs (n=3) and (C) JNK1 phosphorylation (n=4). Data are presented as the mean ± standard error of the mean. ^***P<0.001 vs. resting control and ^###P<0.001 vs. the DMSO-treated group. MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase.