Figure 1.

Nec-1 treatment increases cell viability and reduces cell death in C2C12 cells under conditions of CoCl₂-induced hypoxia. (A) Cell viability was assessed using a CCK8 assay and is expressed as the percentage of the control, ^*P<0.05, compared with the control group. (B) Cells stained with Annexin V-fluorescein isothiocyanate on the x-axis and 7-AAD on the y-axis. Data are expressed as the mean ± standard error of the mean from three independent experiments. ^**P<0.01 and ^***P<0.001, compared with the control group; ^##P<0.01, compared with the CoCl₂ group. CoCl₂, cobalt chloride; 7-AAD, 7-amino-actinomycin D.