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. 2018 Feb 6;41(5):2565–2572. doi: 10.3892/ijmm.2018.3466

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Nec-1 treatment decreases intracellular ROS in C2C12 cells under hypoxia induced by CoCl₂. (A) Flow cytometry images and (B) statistical analysis of ROS-positive cells. The x-axis represents DCF intensity, whereas the y-axis indicates the cell count corresponding to fluorescence intensity. ^***P<0.001, compared with the control group; ^##P<0.01 compared with the CoCl₂ group. ROS, reactive oxygen species; CoCl₂, cobalt chloride.