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. 2018 Feb 20;41(5):2517–2526. doi: 10.3892/ijmm.2018.3506

Figure 6.

Figure 6

Effects of mangiferin on Dex-induced BMP2 downregulation. MC3T3-E1 cells were incubated with 1 µM Dex for 48 h in the absence or presence of mangiferin at the indicated concentrations for 3 h. (A) BMP2 expression was measured by qPCR. (B) Cell lysates were subjected to western blot analysis using a BMP2-specific antibody. (C) Intensity of the protein bands of a typical experiment was semi-quantified using ImageJ 1.41o software. MC3T3-E1 cells were infected with pLVX-BMP2 prior to 1 µM Dex treatment for 48 h. (D) BMP2 expression was measured by qPCR. (E) Cell lysates were subjected to western blot analysis using a BMP2-specific antibody. (F) Intensity of the protein bands of a typical experiment was semi-quantified using ImageJ 1.41o software. +++P<0.001 compared with the control group; ##P<0.01 and ###P<0.001 compared with the Dex treatment group. BMP2, bone morphogenetic protein 2; Dex, dexamethasone; qPCR, quantitative polymerase chain reaction.