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. 2018 Feb 13;41(5):2609–2618. doi: 10.3892/ijmm.2018.3489

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Changed expression levels of hyperpolarization-activated cyclic nucleotide-gated (HCN)1 channel regulatory proteins in spinal cord injury (SCI) rat bladders. Using western blot analysis, the protein expression level was detected of (A) tetratricopeptide repeat-containing Rab8b-interacting protein (Trip8b), (B) filamin A, (C) neural precursor cell-expressed developmentally downregulated (Nedd)4-2 and (D) neuronal restrictive silencing factor (NRSF) in bladders of all three groups. (E) Compared with that in control and sham rat bladders, the protein expression levels of Trip8b, Nedd4-2 and NRSF were significantly increased, while the protein expression level of filamin A was significantly downregulated in SCI rat bladders (^*P<0.05, ^**P<0.01 and ^***P<0.001).