Table I.
Primers used for quantitative RT-PCR.
| Genes | Species | Primer sequences | Accession no. |
|---|---|---|---|
| HCN1 | Rat | F: 5′-CATGCCACAGCTTTGATCCAGTCT-3′ R: 5′-CGCATGTCAGCTGGTAACTTGTG-3′ |
NM_053375 |
| HCN2 | Rat | F: 5′-AAGGGCAACAAGGAGATGAAGC-3′ R: 5′-CGCCGCATCATGGGGTACTC-3′ |
NM_053684 |
| HCN3 | Rat | F: 5′-GCAGCGCATCCACGAGTACTACG-3′ R: 5′-CCCCGGCAGGTGAAGTTAATAATC-3′ |
NM_053685 |
| HCN4 | Rat | F: 5′-CGCTCGCTCTCCACAGGCTG-3′ R: 5′-CGGGGGTGTCTCTGGTGTACTTG-3′ |
NM_021658 |
| GAPDH | Rat | F: 5′-GGCCCCTCTGGAAAGCTGTG-3′ R: 5′-CCAGGCGGCATGTCAGATC-3′ |
NM_017008 |
F, forward; R, reverse; HCN, hyperpolarization-activated cyclic nucleotide-gated channel; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.