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. 2018 Feb 12;41(5):2678–2686. doi: 10.3892/ijmm.2018.3481

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Copyright: © Kobak et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Expression of Desmin in L6 cells with concomitant optimal, reduced or increased iron availability in normoxia and hypoxia. (A) mRNA expression levels of Desmin in L6G8C5 cells. (B) Western blot analysis of respective proteins expression in the cell lysates. (C) Representative images of immunocytochemical staining of Desmin with DAPI as a nuclei marker in L6G8C5 cell lines. Scalebar length, 25 µm. ^*P<0.05; ^***P<0.001. AU, arbitrary units; DFO, reduced iron concentration via deferroxamine; C, control; AFC, increased iron concentration via ammonium ferric citrate.