Figure 4.

Licochalcone A (LicA) induces autophagy through reactive oxygen species (ROS) pathway in hepatocellular carcinoma cells (HCCs). HCCs were pretreated with antioxidant N-Acetyl-L-cysteine (NAC) for 2 h, and treated with 0.1% DMSO or 50 µM LicA for 24 h. (A) The expression level of LC3 was detected by immunoblot. (B) HCCs were transfected with GFP-LC3 plasmid, and counting the LC3-GFP puncta positive cells (at least 5 puncta) with fluorescence microscope. (C) acridine orange (AO) staining for detecting autolysosomes. ^*P<0.05.