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Antioxidant N-Acetyl-L-cysteine (NAC) enhances LicA-induced apoptosis. Hepatocellular carcinoma cells were pretreated with antioxidant NAC for 2 h, and treated with 0.1% DMSO or 50 µM LicA for 24 h. (A) Cell death ratio was measured by Trypan blue staining. (B) Cell viability was measured by cell counting kit-8 (CCK-8) kit. (C) Caspase-3 was measured by immunoblotting. (D) Apoptosis was detected by FACScan flow cytometer. *P<0.05 and **P<0.01.
