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. 2018 Feb 23;41(5):2573–2588. doi: 10.3892/ijmm.2018.3512

Figure 2.

Figure 2

Detailed images of focal adhesion kinase (FAK) inhibitor (Y15) inhibition of cell migration using (A) Transwell assay for 24 h and (C) scratch wound assays within 2 h. (A) Migrating EA.hy926 and HepG2 cells with or without 50 µmol/ml Y15 for 24 h by Transwell assay (×10 magnification); scale bar, 100 µm. (B) Statistical analysis of migrating cells numbers in one field of vision.*P<0.05 vs. controls. The mean ± standard error of three independent experiments are shown. (C) Detailed images of FAK inhibition with 50 µM Y15 blocking cell migration within 2 h. Cell migration of EA.hy926 and HepG2 cells with FAK inhibition within 2 h by scratch wound assay. The dotted lines denote the baseline of the scratch wound; the white arrows indicate the visible migrated cells in the two control groups; scale bar, 100 µm.