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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Mol Microbiol. 2015 Aug 14;98(2):318–328. doi: 10.1111/mmi.13123

Fig. 4.

Fig. 4

VtlR binds to the promoter regions of the small RNA abcR2, as well as three genes bab1_0914, bab2_0512 and bab2_0574 encoding hypothetical proteins.

A. Recombinant VtlR (rVtlR) protein was evaluated for binding to the abcR2 promoter region using electrophoretic mobility shift assays (EMSAs). Increasing concentrations of rVtlR were incubated with radiolabeled abcR2 promoter region DNA. In some binding reactions, either unlabeled specific (abcR2 promoter region DNA) or unlabeled non-specific (abcR1 promoter region DNA) competitor DNA fragments were added to the binding reactions.

B. EMSAs of rVtlR protein binding to the promoter regions of abcR1, abcR2, bab1_0914, bab2_0512 and bab2_0574. Radiolabeled promoter DNA fragments (approximately 200 bp) were either incubated with (+) or without (−) 160 nM rVtlR.