Table 1. Primer.
| Symbol | Gene | Genbank no. | Primer A | Primer B | Fragment length (base pairs) |
|---|---|---|---|---|---|
| Actb | Actin beta | NM_007393.3 | CACCCGCGAGCACAGCTTCTTTG | TGCACATGCCGGAGCCGTTGTC | 116 |
| Aldoa | Aldolase A, fuctose-bisphosphate | NM_001177308.1 | AGGCAGTGGGAGGCAATATCT | TTCTCCTCGGTGTTCTCGGT | 308 |
| Angpt2 | Angiopoietin 2 | NM_007426.4 | ACCTTACAGGACTCACGGGG | TCATGGTTGTGGCCTTGAGC | 264 |
| Ccl2 | Chemokine (C-C motif) ligand 2 | NM_011333.3 | CTGTCATGCTTCTGGGCCTG | AGGCATCACAGTCCGAGTCA | 459 |
| Egr1 | Early growth response 1 | NM_007913.5 | GCCGAGCGAACAACCCTATG | ATTGGTCATGCTCACGAGGC | 226 |
| Eno1 | Enolase 1 | NM_023119.2 | TCTTTCCTTGCTTTGCAGCGA | CCAGAGCAGGCGCAATAGTT | 274 |
| Glut1 (Slc2a1) | Solute carrier family 2 (facilitated glucose transporter), member 1 | NM_011400.3 | CTGGCGGGAGACGCATAGTT | CAAAGCCAGTAGTCAGGCCG | 499 |
| Glut3 (Slc2a3) | Solute carrier family 2 (facilitated glucose transporter), member 3 | NM_011401.4 | AGGTCACTGAATTCCTGGGGT | TCAGCAGTCCCTCACTTGGT | 412 |
| Hif1a | Hypoxia inducible factor 1, alpha subunit | NM_010431.2 | GCGAGAACGAGAAGAAAAAGATGAG | CCCTTTTCTCACTGGGCCAT | 458 |
| Hif2a (Epas1) | Endothelial PAS domain protein 1 | NM_010137.3 | AAGTGCACGGTCACCAACAG | AGGTTGCGGGGGTTGTAGAT | 479 |
| Igfbp3 | Insulin-like growth factor binding protein 3 | NM_008343.2 | AGCCTAAGCACCTACCTCCC | CTTGGAATCGGTCACTCGGT | 126 |
| Il1b | Interleukin 1 beta | NM_008361.3 | CTGCTGGTGTGTGACGTTCCCA | AGGGTGGGTGTGCCGTCTTTC | 254 |
| Il6 | Interleukin 6 | NM_031168.1 | CCGGAGAGGAGACTTCACAGAGGA | TGGATGGTCTTGGTCCTTAGCCAC | 483 |
| Ldha | Lactate dehydrogenase A | NM_010699.2 | TCCATTTAAGGCCCCGCCC | AGCACCAACCCCAACAACTG | 222 |
| Met | MET proto-oncogene | NM_008591.2 | CTGGAGGACAAGACCACCGA | GGCCGTGTAGGACGACATTC | 262 |
| Mmp3 | Matrix metallopeptidase 3 | NM_010809.1 | ATGGGCCTGGAACAGTCTTG | AGTCCTGAGAGATTTGCGCC | 440 |
| Mmp9 | Matrix metallopeptidase 9 | NM_013599.3 | CTTCCCCAAAGACCTGAAAACCT | GGAAAGGCGTGTGCCAGAAG | 489 |
| Nrp2 | Neuropilin 2 | NM_001077403.1 | ACTCCTTTGGGTCATCCGTG | ATCCTCACCTGCAAAAGCTGAT | 211 |
| Pfkl | Phosphofructokinase, liver | NM_008826.4 | TTTTGGAGGTGATGGGACGG | ATAGGCTTTCCATGCCGGTC | 211 |
| Pgk1 | Phosphoglycerate kinase 1 | NM_008828.2 | CATCTCCGGGCCTTTCGACCTCA | TCAGGCATGGGAACACCATCAGGC | 257 |
| Pkm | Pyruvat kinase, muscle | NM_001253883.1 | GTCACTCCACAGACCTCATGG | TACAAGCGTTGCTGGCCTAA | 466 |
| Ppbp (Cxcl7) | Pro-platelet basic protein, Chemokine (C-X-C motif) Ligand 7 | NM_023785.2 | CAGCCTCACGTTGTTCCCT | TCAAACCCTCAACCCTTCCTG | 457 |
| Tgfb1 | Transforming growth factor, beta 1 | NM_011577.1 | CCGCGTGCTAATGGTGGACCG | GCCCTGTATTCCGTCTCCTTGGTT | 322 |
| Tgfb2 | Transforming growth factor, beta 2 | NM_009367.3 | TGGCCGAGCAGCGGATTGAACT | ACGTCGAAGGAGAGCCATTCACCC | 127 |
| Tgfb3 | Transforming growth factor, beta 3 | NM_009368.3 | GCCAACTTCTGCTCAGGCCC | GCCTCTAGGGTGAGGTCTGTCG | 305 |
| Timp4 | Timp metallopeptidase inhibitor 4 | NM_080639.3 | TAAAGGGTTCGAGAAGGCCAAG | CAGAGACACTCATTGGGGGC | 291 |
| Tnf | Tumor necrosis factor | NM_013693.2 | ATCCGCGACGTGGAACTGGC | TCGGGGCAGCCTTGTCCCTT | 431 |
| Vegfa | Vascular endothelial growth factor A | NM_009505.4 | CGGGCCTCGGTTCCAGAAGG | TCGGACGGCAGTAGCTTCGC | 280 |
| Vegfr1 (Flt1) | Fms-related tyrosine kinase 1 | NM_010228.3 | CAAGAGCGATGTGTGGTCCT | TGGAGTTCGGTGAAAGCTCC | 473 |
| Vim | Vimentin | NM_011701.4 | GCTGCTGGAAGGCGAGGAGA | TTCTTGCTGGTACTGCACTGTTGC | 238 |
Primer pairs used for qPCR.