Table 4. Age-dependent differentially methylated CpGs/regions detected by conserved Illumina human MethylationEPIC probes and by MBD-seq.
| EPIC1 | MBD-seq1 | ||||||
|---|---|---|---|---|---|---|---|
| ProbeID | Gene2 | Region2 | Position (mm10) 3 | Coef. | Age (P) | logFC | Age (P) |
| cg08949408 | C1QL3 | Body; Island | chr2:13.01 | 0.32 | 0.001 | 3.3 | 1.3E-10 |
| cg10444382 | RFX4 | Body; Island | chr10:84.76 | 0.24 | 9.4E-04 | 2.9 | 2.5E-08 |
| cg22384902 | LRRC4; SND1 | TSS1500; island | chr6:28.83 | 0.22 | 0.009 | 2.0 | 2.0E-06 |
| cg06945399 | LRRC4; SND1 | TSS200; Island | chr6:28.83 | 0.18 | 0.057 | 1.5 | 2.2E-05 |
| cg23398076 | MEIS1 | Body; Shelf | chr11:19.02 | 0.13 | 0.007 | 1.5 | 2.4E-05 |
| cg05393688 | TSC22D1 | Body; Shore | chr14:76.51 | 0.17 | 0.005 | 1.5 | 2.8E-05 |
| cg20563498 | USP35 | Body; Shelf | chr7:97.32 | -0.02 | 0.27 | 1.1 | 3.2E-05 |
1These are age-dependent differentially methylated CpG regions discovered in the MBD-seq at an FDR of 10%; replicated for the corresponding CpG in the EPIC microarray at an uncorrected p-value cutoff of 0.05. Coef. is the linear regression coefficient (i.e., change in methylation β-value from young to old). LogFC is log2 fold change in methylation from young to old.
2CpG location in relation to gene features and CpG region based in probe annotations for the human methylation microarray; gene feature annotations are the same for the corresponding regions in the mouse genome.
3Chromosome and Megabase coordinate based on mm10 mouse reference genome