Table 1. Differentially expressed proteases in the biopsy supernatants of patients with IBS, UC, and controls.
IBS vs. HC | UC vs. HC | IBS vs. UC | |||||||
---|---|---|---|---|---|---|---|---|---|
UniProt ID_HUMAN | Name | Localization | Function | log10 ratio | P value | log10 ratio | P value | log10 ratio | P value |
1. Q96QL8 | Elastase 3A | Extracellular | Serine type endopeptidase with some elastolytic action, digestive function, intestinal cholesterol metabolism | 0.63 | 0.026 | 0.22 | 0.870 | 0.41 | 0.025 |
2. A8MTQ9 | Chymotrypsin-C | Extracellular | Serine type endopeptidase (pancreatic), some elastolytic action, chymotrypsin-like function, activation and degradation of trypsinogens and procarboxypeptidases | 0.60 | 0.014 | 0.21 | 0.977 | 0.39 | 0.003 |
3. D3DPS0 | Proteasome subunit beta type-2 | Cytoplasm | Multicatalytic threonine peptidase, caspase-,trypsin-, and chymotryosin-like activity, antigen processing and presentation via MHC class I | 0.38 | 0.006 | 0.10 | 0.500 | 0.28 | 0.034 |
4. CO3 | Complement C3 (unspecified isoform) | Extracellular | Molecule system including one/several serine proteases, activation of complement system, antimicrobial action. | 0.29 | 0.016 | -0.15 | 0.366 | 0.44 | 0.002 |
5. B4DJQ8 | Cathepsin C | Cytoplasm | Cysteine endopeptidase controlling activation of serine proteases including elastase in inflammatory cells | 1.18 | 0.001 | 1.10 | 0.007 | 0.07 | 0.493 |
6. B3KQK4 | Cathepsin L1 | Cytoplasm | Cysteine endopeptidase with collagen and elastase as substrates; control of neutrophil elastase activity | 0.51 | 0.005 | 0.29 | 0.309 | 0.22 | 0.081 |
7. DNPEP | Aspartyl aminopeptidase | Cytoplasm | Intracellular protein and peptide metabolism | 0.37 | 0.006 | 0.24 | 0.219 | 0.14 | 0.276 |
8. D3DW86 | Proteasome subunit alpha type-4 | Cytoplasm | Multicatalytic threonine peptidase, immune defense, host-virus interaction | 0.16 | 0.017 | 0.05 | 0.133 | 0.11 | 0.113 |
9. B2R7F8 | Plasminogen | Extracellular | Serine type endopeptidase; cell surface; precursor of plasmin; pro inflammatory; immune defense | 0.27 | 0.450 | -1.33 | 0.073 | 1.60 | 0.006 |
10. CO3 | Complement C3 | Extracellular | Molecule system including one/several serine proteases, activation of complement system, antimicrobial action. | -0.10 | 0.823 | -1.53 | 0.014 | 1.43 | 0.004 |
11. Q5U000 | Cathepsin Z | Cytoplasm | Cysteine carboxypeptidase with broad specificity, immune processes | -0.55 | 0.013 | -0.22 | 0.034 | -0.33 | 0.569 |
12. B2CIS9 | Caspase 14 | Cytoplasm | Apoptosis-related cysteine peptidase | -0.49 | 0.006 | -0.18 | 0.091 | -0.31 | 0.086 |
13. Q6IAT9 | Proteasome subunit beta type-6 | Cytoplasm | Multicatalytic threonine peptidase, ATP-dependent proteolytic activity, caspase-like activity, immune defense | -0.40 | 0.019 | -0.10 | 0.107 | -0.31 | 0.221 |
14. Q5QNR8 | Proteasome subunit beta type-8 | Cytoplasm | Multicatalytic threonine peptidase, ATP-dependent proteolytic activity, immune defense, facilitates apoptosis | -0.28 | 0.007 | -0.16 | 0.043 | -0.12 | 0.315 |
15. A8K071 | X-prolyl aminopeptidase 1 | Cytoplasm | Metalloaminopeptidase; degradation of tachykinins, neuropeptides, and peptide hormones | -0.62 | 0.016 | -0.28 | 0.640 | -0.35 | 0.037 |
16. A8K6H1 | Endoplasmic reticulum aminopeptidase 1 | Extracellular | Zinc metallopeptidase, ubiquitous, immune defense, presentation of MHC class I presentation | -0.26 | 0.046 | 0.15 | 0.519 | -0.41 | 0.012 |
17. Q6IBC3 | Cathepsin H | Cytoplasm | Cysteine endo-and exopeptidase, enkephalin and galanin neurotransmitter production | -0.31 | 0.094 | 0.14 | 0.338 | -0.44 | 0.020 |
P values for the F-test between all three groups were < 0.036; UniProt ID: universal protein database identification number: log10 ratio: differences in the log10 protein abundance; listed are those peptidases showing a significant difference between each of two study group
1–4 higher abundance in IBS versus HC and UC. 5 higher abundance in IBS versus HC and UC versus HC. 6–8 higher abundance only in IBS versus HC. 9–10 higher abundance in IBS versus UC. 11–14 lower abundance in IBS versus HC. 15–16 lower abundance in IBS versus HC and UC. 17 lower abundance in IBS versus UC. Data on function from Uniprot