Fig. 5.

Assessing intestinal integrity of ileum 21 dpc in sentinel, control and vaccinate pig groups. Goblet cells were identified using PAS staining. IF was used to detect mucin-2 (MUC2), intestinal alkaline phosphatase (IAP), cleaved-caspase-3 (CASP3) and calprotectin (CP). Anti-MUC2 and anti-calprotectin antibodies were detected using Alexa-647-conjugated secondary antibody (purple). Anti-cleaved Caspase-3 antibody was detected using Alexa-488-conjugated secondary antibody (green). Intestinal alkaline phosphatase was detected as described in materials and methods. DAPI (blue) was used to counterstain the nuclei. Scale bar: 20 µM. Representative images are shown from animals with median results for each staining method and group. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)