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Vinculin controls zonula occludens (ZO)-1 localization upon injury. Co- immunoprecipitation of control and Pod-Vcl-KO podocytes shows vinculin association with ZO-1 in controls (ctrl), but not in IgG or vinculin KO podocytes (A). Representative XY and XZ images show that ZO-1- colocalization with membrane marker E-cadherin is reduced in Pod-Vcl-KO podocytes after lipopolysaccharide (LPS) and protamine sulfate (PS) stimulation (B).
