Figure 6.

Re-expression of green fluorescent protein (GFP)–Vcl in Pod-Vcl-KO podocytes rescues zonula occludens (ZO)-1 mislocalization after either lipopolysaccharide (LPS) or protamine sulfate (PS) treatment. Representative image of Pod-Vcl-KO primary podocytes re-expressing GFP-vinculin shows that zonula occludens (ZO)-1 is at the cell–cell junctions, and this persists after the LPS challenge (white arrowheads). Note the ZO-1 mislocalization in adjacent, untransfected Pod-Vcl-KO podocytes serving as an internal control (arrowhead). Bar = 10 μm. GFP alone transfection was also performed as a control (ctrl). Note that the bottom panel stimulated with LPS is at x100 (a). Quantitative analysis of the redistribution of ZO-1 is represented by comparing the cytoplasmic to the total cellular immunofluorescence intensity after either LPS or PS treatment in mouse GFP–vinculin expressing and nonexpressing primary podocytes; n = 3; *P < 0.05 (b). To optimize viewing of this image, please see the online version of this article at www.kidney-international.org.