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. 2018 Jan 28;22(4):1031–1039. doi: 10.1016/j.celrep.2017.12.086

Figure 3.

Figure 3

RNAPII Accumulates at the PPP Sites of Actively Transcribed Genes

(A) ChIP analyses with RNAPII antibody (N20) of the ACTB and GAPDH genes in EUFA423 B2 and EUFA423 cells. Fold change relative to IgG isotype control is plotted. Plots show the mean ± SEM from three independent experiments. The 2-way ANOVA test was performed to determine statistical significance (∗p < 0.05).

(B) ChIP analyses of RNAPII Ser5P in the ACTB and GAPDH genes in EUFA423 B2 and EUFA423 cells. Data were normalized to input (2%). Plots show the mean ± SEM from three independent experiments; the 2-way ANOVA test was performed as above.