Skip to main content
. 2018 Jan 28;22(4):1031–1039. doi: 10.1016/j.celrep.2017.12.086

Figure 5.

Figure 5

BRCA2 Inactivation Diminishes PAF1 Recruitment at PPP Sites and Subsequent H2B K120 Ubiquitination

(A and B) ChIP analyses with PAF1 antibody of the ACTB and GAPDH genes in HeLa Kyoto cells 72 hr after transfection with siCtrl or siBRCA2 (A) and EUFA423 B2 and EUFA423 cells (B).

(C and D) ChIP analyses with H2B K120ub antibody of GAPDH gene in HeLa Kyoto cells 72 hr after transfection with siCtrl or siBRCA2 cells (C) and EUFA423 B2 and EUFA423 cells (D). Fold change relative to the IgG isotype control antibody control is plotted.

Plots show the mean ± SEM from three independent experiments. Data were normalized to input (2%). The 2-way ANOVA test was performed for all pairwise comparisons to determine statistical significance. Statistically significant differences are indicated (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001).