Figure 3.

Starvation Promotes Episodes of Global Neural Inactivity

(A) Maximum intensity projections across the indicated time periods of the brain-wide imaging recording shown in (C). Selected neuronal classes are labeled.

(B and C) Heat plots of representative brain-wide imaging recordings for WT fasted (B) and WT starved (C) worms. O₂ stimulation as indicated. Rows are NLS-GCaMP5K fluorescence time series (ΔF/F₀) of segmented head neurons, sorted by correlations. Example traces are shown below: reversal interneuron AVA, forward motor neurons VB02 or VB01, turning head motor neurons RIV, SMDV, and SMDD, and GABAergic neurons RIS and RMED/V. Grey shadings indicate quiescence periods.

(D and E) Left: brain state ethograms showing quiescence episodes of worms in brain-wide imaging recordings with O₂ stimulation as indicated. One row corresponds to one recording, each a different animal. Right: traces show mean (±SEM) fraction of time spent quiescent in 1-min bins, across all corresponding datasets shown on the left. n indicates number of recordings. (E) Same as (D) but at constant 21% O₂. Green traces in (D) and (E) on the right are the same data.

See Figures S4 and S5.