Figure 3. Tissue-specific FITC mAbs modulate mAbCAR T cell homing and function in vivo.

(A) Targeting mAbCAR T cells regulates homing. The graph shows bioluminescent signal from allogeneic luc⁺ untransfected Tcons (black), luc⁺ SDF1-mAbCAR Tcons (blue), or luc⁺ MAdCAM1-mAbCAR Tcons (red) at day +4, +7, and +12 after transfer in mice that received TCD BM at day 0. One representative of three consecutive experiments is presented; 2–4 mice/group were used in each experiment. ANOVA test with Bonferroni post-test; mean ± SEM; ***P < 0.001. (B) Targeting mAbCAR T cells alters localization. Representative bioluminescent images of mice that received luc⁺ untransfected Tcons, luc⁺ SDF1-mAbCAR Tcons, or luc⁺ MAdCAM1-mAbCAR Tcons at day +12 after transfer. Data are representative of 1 of 3 consecutive experiments. (C) Targeting mAbCAR T cells modulates GvHD. GvHD score over time of recipient mice that received allogeneic untransfected Tcons, SDF1-mAbCAR Tcons, or MAdCAM1-mAbCAR Tcons. One representative of three independent experiments is presented. ANOVA test with Bonferroni post-test; mean ± SEM; ***P < 0.001. (D) mAbCAR T cells mediate graft-versus-tumor effects. Tumor growth analyzed by BLI in lethally irradiated BALB/c mice that received allogeneic C57BL/6 TCD BM and luc⁺ A20 alone (black), luc⁺ A20 and allogeneic isotype-mAbCAR Tcons (gray), or luc⁺ A20 and allogeneic SDF1-mAbCAR Tcons (blue). One representative of two consecutive experiments is reported; at least 3 mice/group were used. Two-tailed Student’s t test; mean ± SEM; ***P < 0.001. (E) mAbCAR T cells eliminate tumor in vivo. Representative bioluminescent images of lethally irradiated BALB/c mice that received allogeneic C57BL/6 TCD BM and luc⁺ A20 alone, luc⁺ A20 and isotype-mAbCAR Tcons, or luc⁺ A20 and SDF1-mAbCAR Tcons at day +7, +14, and +21 after transplant. One representative of two consecutive experiments is reported; at least 3 mice/group were used. (F) SDF1-directed mAbCAR T cells enhance survival. Survival of lethally irradiated BALB/c mice that received irradiation alone (black circle), allogeneic C57BL/6 TCD BM (black squares), TCD BM + luc⁺ A20 alone (white squares in black), TCD BM + luc⁺ A20 + allogeneic isotype-mAbCAR Tcons (red triangles), TCD BM + luc⁺ A20 + allogeneic SDF1-mAbCAR Tcons (white circles in blue). One representative of two consecutive experiments is reported; at least 3 mice/group were used. Log-rank survival test; *P < 0.05 for differences between the groups that received luc⁺ A20 alone and luc⁺ A20 + allogeneic SDF1-mAbCAR Tcons.