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. 2017 Oct 19;2(20):e95085. doi: 10.1172/jci.insight.95085

Figure 6. Overexpression of Jarid2 and Notch1 downregulation results in developmental mortality in the Drosophila maternal diabetes and gene-environment interaction model.

Figure 6

(A) Glucose and (B) trehalose levels measured in hemolymph of adult WT flies with standard and diabetic (0.2 g/ml) maternal diet, along with WT progeny flies and those overexpressing heart-specific Jarid2, Numb, and Notch1 RNAi and Jarid2 RNAi transgenes (n = 6 using 50 adult flies) (mean ± SEM; *P < 0.05 by 2-tailed Student’s t test). (C) Survival curves for adult progenies exposed to nondiabetic and diabetic maternal diet overexpressing heart-specific Jarid2 RNAi transgenes compared with controls. (D) Adult fly heart phenotypes in progeny of nondiabetic and diabetic mothers. Cardiomyocyte muscle fiber actin (phalloidin, red) and pericardin (blue) were stained. Red dotted lines delineate heart tube. Scale bar: 5 μm. Quantification of (E) heart tube pericardin deposition and (F) cardiomyocyte actin fiber density in adult progeny of nondiabetic and diabetic mothers (n = 6; mean ± SD; *P < 0.05, 2-tailed Student’s t test).