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. 2017 Oct 19;2(20):e94375. doi: 10.1172/jci.insight.94375

Figure 5. RNA in situ hybdridization for the alternative Klotho mRNA transcript in HK-2 cells reveals colocalization with some P bodies.

Figure 5

(A) RNA in situ hybridization for the alternative Klotho mRNA transcript indicates expression in HK-2 cells. (B) Control cells hybridized without probe. Insets magnify individual cells. (C) Immunofluorescence for Dcp1a identifies P bodies in HK-2 cells, (D) merged with bright-field image. (E) Immunofluorescence without primary antibody, (F) merged with bright-field image. (G) Immunofluorescence for Dcp1a in HK-2 cells, (H) merged with bright-field image. (I) RNA ISH reveals that some P bodies in the HK-2 cells in G and H colocalize with the signal for the alternative Klotho mRNA transcript (arrows). Images depicted in panels A, B, and I were digitally contrast-enhanced to 150% in Microsoft Powerpoint. Original magnifications are 400×; insets are 2-fold the magnification of A and B.