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. 2017 Oct 19;2(20):e96783. doi: 10.1172/jci.insight.96783

Figure 2. Testing the ability of an immunopurified goat IgG against chicken lipoprotein lipase (cLPL) to bind to cLPL in cultured cells.

Figure 2

CHO pgsA-745 cells were transiently transfected with V5-tagged cLPL. Immunocytochemistry studies were performed on fixed and permeabilized cells with a goat antibody against cLPL and a mouse monoclonal antibody against the V5 tag. Binding of the primary antibodies was detected with an Alexa Fluor 555–conjugated donkey anti–goat IgG (red) and an Alexa Fluor 488–conjugated donkey anti–mouse IgG (green). As experimental controls, we included transfected cells that had been incubated with secondary antibodies alone and cells that had been incubated with the anti-cLPL antibody alone. DNA was stained with DAPI (blue). (A) Immunocytochemistry studies on cells that had been fixed with methanol. (B) Studies of cells fixed with paraformaldehyde (PFA). Scale bars: 50 μm.