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. 2017 Oct 19;2(20):e96783. doi: 10.1172/jci.insight.96783

Figure 3. LPL in chicken tissues is associated with capillaries.

Figure 3

Frozen sections from chicken white adipose tissue (WAT) and heart were stained with a rabbit antibody against von Willebrand factor (vWF), followed by an Alexa Fluor 488–conjugated donkey anti–rabbit IgG (green) and an Alexa Fluor 555–conjugated goat anti–chicken lipoprotein lipase (anti-cLPL) IgG (red). DNA was stained with DAPI (blue). (A) Sections of heart stained with both cLPL and vWF antibodies, revealing colocalization of cLPL and vWF in capillaries. As controls, we examined a section incubated with secondary antibodies alone and a section stained with the vWF antibody and nonimmune goat IgG. (B) An independent chicken immunohistochemistry study in which sections of WAT and heart were stained with cLPL and vWF antibodies. Scale bars: 50 μm.