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. 2018 Feb 26;8(2):2045894018764171. doi: 10.1177/2045894018764171

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© The Author(s) 2018

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 1. — nmMLCK1 Y⁴⁶⁴ phosphorylation and cellular localization upon S1P challenge. Human pulmonary microvascular ECs were transfected with EGFP-nmMLCK1 constructs with WT, S147P, or 3SNP. Then ECs were challenged with S1P (1 μM) for 2 or 5 min. Immunofluorescence assays were performed to visualize nmMLCK Y⁴⁶⁴ phosphorylation (red), F-actin (blue), and transfected EGFP-nmMLCK1 (green). These representative images were selected from > 5 independent assays.