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. 2018 Feb 26;8(2):2045894018764171. doi: 10.1177/2045894018764171

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© The Author(s) 2018

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 2. — Effects of genetic variants on nmMLCK1 Y⁴⁶⁴ phosphorylation post S1P challenge. Human pulmonary microvascular ECs were transfected with EGFP-nmMLCK1 constructs with WT, S147P, or 3SNP. Then ECs were challenged with S1P (1 μM) for 2–15 min. Immunofluorescence assays were performed to visualize nmMLCK1 Y⁴⁶⁴ phosphorylation. Relative nmMLCK1 Y⁴⁶⁴ phosphorylation levels in ECs were quantified. n = 30. *P < 0.05 compared to unstimulated WT EGFP-nmMLCK1 (the first white bar). #P < 0.05 compared to WT EGFP-nmMLCK1 group at the same time point of S1P challenge (the white bar of each time point).