Figure 6.
Release of chitin oligosaccharides during swollen chitin hydrolysis by Chitinase Chi1. Swollen chitin (0.45% (w/v)) was incubated with purified Chitinase Chi1 (100 nM) at 50 °C in 1 mL of 0.05 M sodium phosphate buffer pH 6.0. Aliquots were taken at different time intervals, and the hydrolysis products were analyzed by HPAEC. Detected products were GlcNAc (cross), (GlcNAc)2 (triangle), and (GlcNAc)3 (square). Soft lines are only drawn as visual aids. The error bars represent the range of duplicate experiments.