Figure 7.
Hydrolysis of chitin oligosaccharides (GlcNAc)3 (A), (GlcNAc)4 (B), (GlcNAc)5 (C), and (GlcNAc)6 (D) by Chitinase Chi1. Chitin oligosaccharides (2 mM) in 0.5 mL of 0.05 M sodium phosphate buffer pH 6.0 were incubated with purified Chitinase Chi1 (25 nM). Aliquots were taken at different time intervals, and the hydrolysis products were analyzed by HPAEC. Detected products were GlcNAc (star), (GlcNAc)2 (triangle), (GlcNAc)3 (square), (GlcNAc)4 (circle), (GlcNAc)5 (cross), and (GlcNAc)6 (diamond). Experimental work performed in duplicates and the standard deviation was less than 5%.