Table 1.
Summary of diagnostic results obtained using both the hot-start gel-based PCR and the UPL real-time PCR assay across the three sampling strategies between 2011 and 2013
| Year | Month | Reason for sampling | Sampling location | Number of domestic pigs sampled | ASFV DNA detection results | Genotyped | |
|---|---|---|---|---|---|---|---|
| (Town/District) | Conventional PCR | UPL PCR | |||||
| No. of positive pigs | No. of positive pigs | ||||||
| 2011 | Sep | Suspected outbreak | Kisauni | 2 | 2 | 2 | * |
| Dec | Suspected outbreak | Nairobi | 2 | 0 | 0 | ||
| Suspected outbreak | Kiambu | 2 | 2 | 2 | * | ||
| 2012 | Jan | Suspected outbreak | Kiambu | 7 | 1 | 1 | * |
| Feb | Suspected outbreak | Kiambu | 1 | 0 | 0 | ||
| Suspected outbreak | Nyeri | 1 | 0 | 1 | |||
| Suspected outbreak | Machakos | 1 | 1 | 1 | * | ||
| Mar | Suspected outbreak | Athi river | 2 | 2 | 2 | * | |
| Suspected outbreak | Thika | 1 | 1 | 1 | |||
| Suspected outbreak | Kikuyu | 1 | 1 | 1 | |||
| Apr | Suspected outbreak | Nakuru | 1 | 1 | 1 | ||
| May | Suspected outbreak | Kiambu | 2 | 1 | 1 | * | |
| Jun | Suspected outbreak | Nairobi | 3 | 3 | 3 | * | |
| Suspected outbreak | Busia (Burumba) | 5 | 1 | 1 | * | ||
| Suspected outbreak | Kiambu | 2 | 1 | 1 | * | ||
| Suspected outbreak | Kajiado | 1 | 1 | 1 | |||
| Jul | Suspected outbreak | Kiambu | 2 | 0 | 0 | ||
| Suspected outbreak | Nairobi | 1 | 0 | 0 | |||
| Aug | Suspected outbreak | Kiambu | 2 | 1 | 1 | ||
| Sep | Suspected outbreak | Kiambu | 21 | 1 | 1 | * | |
| Oct | Suspected outbreak | Kajiado | 2 | 3 | 3 | ||
| Suspected outbreak | Kiambu | 1 | 0 | 0 | |||
| Suspected outbreak | Limuru | 1 | 1 | 1 | |||
| Suspected outbreak | Busia (Sigalame) | 1 | 1 | 1 | * | ||
| Nov | Suspected outbreak | Busia (Ameri) | 1 | 1 | 1 | ||
| Dec | Suspected outbreak | Nairobi | 1 | 0 | 0 | ||
| Suspected outbreak | Uasin Gishu | 8 | 0 | 1 | |||
| Dec | Suspected outbreak | Muranga | 1 | 1 | 1 | * | |
| July–October | Endemic ASF districts | Teso and Busia Districts | 605 | 0 | 0 | ||
| 2013 | Jan | Endemic ASF districts | Teso, Busia, & Eastern Uganda | 125 | 2 | 2 | * |
| Apr | Suspected outbreak | Kiambu | 2 | 1 | 1 | * | |
| Suspected outbreak | Kirinyaga | 2 | 0 | 0 | |||
| May | Abattoirs | Busia | 24 | 0 | 0 | ||
| Jun | Suspected outbreak | Machakos | 1 | 1 | 1 | ||
| Suspected outbreak | Muranga | 1 | 1 | 1 | |||
| Suspected outbreak | Kiambu | 1 | 0 | 0 | |||
| Jul | Endemic ASF districts | Teso, Busia, & Eastern Uganda | 87 | 4 | 4 | * | |
| Suspected outbreak | Kiambu | 1 | 1 | 1 | * | ||
| Suspected outbreak | Kirinyaga | 2 | 1 | 1 | * | ||
| Aug | Suspected outbreak | Nakuru | 3 | 1 | 1 | * | |
| Suspected outbreak | Kiambu | 2 | 1 | 1 | * | ||
| Sep | Suspected outbreak | Nyadorera (Nyanza) | 1 | 1 | 1 | * | |
| Suspected outbreak | Kiambu | 3 | 1 | 1 | * | ||
| Suspected outbreak | Nairobi | 1 | 1 | 1 | |||
| Oct | Suspected outbreak | Busia | 1 | 1 | 1 | * | |
| Suspected outbreak | Kajiado | 2 | 0 | 0 | |||
| Suspected outbreak | Kiambu | 1 | 0 | 0 | |||
| Abattoirs | Busia | 16 | 5 | 5 | * | ||
| Nov | Suspected outbreak | Kiambu | 4 | 1 | 1 | * | |
| Dec | Suspected outbreak | Kakamega | 5 | 1 | 1 | * | |
| Suspected outbreak | Kiambu | 1 | 1 | 1 | * | ||
Only three suspected outbreaks were reported between September 2011 (when the study began) and December 2011. Domestic pigs selected for genotyping are indicated by *