Figure S1.

Accumulation and Positioning of cDC1 in COX-Deficient Tumors, Related to Figure 1

WT mice were injected s.c. with 2x10⁶ 4T1 breast cancer cells, CT26 colorectal cancer cells or BRAF^V600E melanoma cells. Tumors were excised 4 days later.

(A and B) Immunofluorescence images of parental 4T1 or Ptgs1/Ptgs2^−/− 4T1 tumors (A) or WT CT26 or Ptgs2^−/− CT26 tumors (B). Upper panels show original images, lower panels show visualization of CD103⁺ cDC1 localization by surface reconstruction. Scale bar 100μm. Images are representative of individual tumors from 5-6 mice in two independent experiments. The dashed lines indicate the tumor margin, arrows indicate multicellular clusters of cDC1.

(C and D) Quantification of intratumoral cDC1 in immunofluorescent images of 4T1 tumors (C) or CT26 tumors (D). Each circle represents data from one individual tumor. Data are mean ± SEM and were pooled from two independent experiments.

(E) Distance analysis based on (A).

(F) Distance analysis based on (B). Line indicates mean value, ^∗∗p < 0.01, ^∗∗∗p < 0.001.