Figure 1.

27HC signalling pathway is expressed in endometrial cancer and altered with disease severity. The expression of CYP7B1, CYP27A1, NR1H3 (LXRα) and NR1H2 (LXRβ) relative to internal control gene CYC was assessed by qPCR in postmenopausal control endometrium (PM Ctrl) and in endometrial cancer tissue homogenates from well-, moderately- and poorly differentiated endometrial adenocarcinomas. Relative expression of mRNAs encoding CYP7B1 (A) were decreased in poorly differentiated cancers compared to moderately differentiated cancers but CYP27A1 was not significantly different (B). Relative expression of mRNAs encoding NR1H3 (C; LXRα) were significantly decreased in moderately differentiated cancers compared to postmenopausal control tissues whilst NR1H2 (LXRβ) was not significantly different (D). *P < 0.05, **P < 0.01. Kruskal–Wallis test with multiple comparisons. PM, n = 9; Well n = 12–30; Mod, n = 42–64; Poor, n = 23–32. All data are presented as mean ± s.e.m.