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. 2018 Feb 13;22(7):1657–1665. doi: 10.1016/j.celrep.2018.01.054

Figure 1.

Figure 1

A Diurnal Pattern of Basal Thermotolerance Correlates with a Diurnal Pattern of Heat-Associated Gene Expression

(A) Basal thermotolerance of WT seedlings over a diurnal time course. Error bars are ±SEM (n = 3), where 81 seedlings were scored per time point and temperature for three independent experiments.

(B) Heatmap of the expression of genes in cluster 13-1-1. Transcriptomes shown from plants grown in short days (8 hr light, 16 hr dark) at constant 17°C, 22°C, or 27°C. Black bars below heatmap indicate samples taken in the dark (ZT−4, ZT0, ZT12, and ZT16) and white bars indicate samples taken in the light (ZT1, ZT2, ZT4, and ZT8). In heat maps, low to high expression (Z scores) is shown as blue to red.

(C–F) Expression of HSP70 in WT seedlings assayed by qRT-PCR.

(C) Plants grown at 17°C, 22°C, and 27°C. Error bars are ± the range of two measurements (n = 2) or SEM (n = 3 to 4).

(D) Seedlings entrained in short days at 27°C and shifted to constant light or constant dark at ZT8 of the seventh day after germination. Plants were sampled starting at ZT−4 on the eighth day. Error bars are ± the range of two measurements.

(E and F) Before and after a 30-min shift to 45°C in seedlings grown at 17°C (E) and 27°C (F).

(G) Induction ratios (expression after shift/expression before shift) of HSP70 expression after the temperature shifts in (F) and (G). Error bars are ±SEM (n = 3).