Fig. 3.

Analysis of CreA-mediated carbon catabolite repression on pectinase genes. a Growth phenotype of the PNRRL3_03144-amdS (JC1.5), ∆creA PNRRL3_03144-amdS (JN29.2), Ppgx28B-amdS (JC3.6) and ∆creA Ppgx28B-amdS (JN31.3) strains on solid MM containing 50 mM fructose, 50 mM GA, or 50 mM GA with increasing amounts of fructose after 7 days at 30 °C. All plates contain 10 mM acetamide as the sole nitrogen source. b PGA plate assay. The reference (MA234.1), OEgaaR (EA21.6), and ∆creAOEgaaR (TK2.1) strains were grown in MM containing 50 mM fructose for 36 h, and serial dilutions of culture supernatants were spotted on PGA plates. Dilution factors are indicated