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Principle of the proposed method to discriminate between external DNA (exDNA) fractions and the internal DNA (iDNA) of the total DNA pool (metagenome). fDNA free DNA, wbDNA weakly bound DNA, tbDNA tightly bound DNA. Ratio-forming (e.g. exDNA:iDNA) is intended as proxy of microbial activity. Here, exDNA is the sum of the fDNA, wbDNA, and tbDNA fractions
