Fig. 3.

Phylogeny of cellobiose dehydrogenase (CDH); AA3_1 genes form three well-supported classes with distinct molecular and catalytic properties. The phylogenetic subtree was calculated using the flavin domains of 21 CDH sequences representative for CDH class I (6 sequences, 4 characterized), class II (9 sequences, 9 characterized), and class III (6 sequences, 0 characterized). The tree was rooted within AA3 family enzymes (not shown). Sequences were aligned using M-coffee (Wallace et al. 2006) with default settings. Phylogeny was inferred using PhyML (Guindon et al. 2010) and the Whelan and Goldman (WAG) amino acid substitution model (Whelan and Goldman 2001). Branch support was calculated by 100 bootstrap repetitions (values displayed in percent). The tree was visualized in MEGA7 (Kumar et al. 2016)