Table III.
Conditions Tested for Compatibility with Caco-2 Cells
| Condition | |
|---|---|
| Buffer | |
| HBSS (pH 6.5) | Control |
| Digestion buffer | Simulated intestinal fluid containing bile salts and phospholipids |
| Excipients | |
| 1.25% (w/v) | Highest concentration of a single excipient used during in vitro lipolysis (Table II) |
| 0.625% (w/v) | Common concentration of single excipient used during in vitro lipolysis (Table II) |
| 0.125% (w/v) | Common concentration of single excipient used during in vitro lipolysis (Table II) |
| LBF | |
| 2.5% (w/v) | Common LBF concentration used during in vitro lipolysis (17), formulations describe in Table II |
| Enzyme | |
| Pancreatic enzyme (900 USPU/mL) | Common concentration of pancreatic enzyme added to in vitro lipolysis experiments (17) |
| Immobilized enzyme (125 PLU/mL) | Concentration of immobilized enzyme required for digestion of LBF |
| FFA | |
| Caprylic acid (87 mM) | Highest concentration of MC FFA released in previous digestions (17) |
| Oleic acid (37.5 mM) | Highest concentration of LC FFA released in previous digestions (17) |
| Digested LBF | |
| LBF digested with immobilized enzyme for 60 min | Common concentration of LBF and time span of in vitro lipolysis experiments digested with an enzyme concentration compatible with Caco-2 cells |