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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Cell Stem Cell. 2017 Sep 28;21(4):489–501.e7. doi: 10.1016/j.stem.2017.08.011

Figure 7. Non-redundant Role of Jak1 Signaling in HSCs.

Figure 7

(A) White blood cell (WBC) counts of recipient mice transplanted with whole bone marrow cells. *p < 0.05. n = 5.

(B) Schematic diagram showing competitive bone marrow transplant design.

(C) Percentage (mean ± SEM) of CD45.1 versus CD45.2 chimerism in the PB of recipients at 1, 2, 3, and 4 months after poly(I:C) administration. Pre-poly(I:C) indicates the ratio of CD45.1 to CD45.2 bone marrow cells in recipient mice before gene deletion. n = 5. *p < 0.05.

(D) Donor chimerism within the LSK compartment in the bone marrow of recipient mice at 16 weeks post-poly(I:C) injection. n = 5. *p < 0.05. See also Figure S7.

(E and F) Percentage (mean ± SEM) of donor-derived total CD45.2 cells in the PB of recipient animals. (E) Secondary transplant, (F) tertiary transplants. n = 5 for each genotype. *p < 0.05. ns, not statistical significant. Only genotypes Jak2V617F and Jak1fl/fl,Jak2V617F were serially transplanted into tertiary recipients.