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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: J Am Chem Soc. 2017 Aug 15;139(34):11646–11649. doi: 10.1021/jacs.7b04159

Figure 4.

Figure 4

(A) SDS-PAGE mNisA ring variants incorporating 1. Lane 1: ring A, lane 2: ring B, lane 3: ring C. (B-D) QTOF-ESI for mNisA ring variants incorporating 1: (B) Ser3TAG, ring A. (C) Thr8TAG, ring B. (D) Thr13TAG, ring C. (E)13C-NMR of labeled 1 (red) and 2 (green). (F) 13C-NMR mNisA Cys to Ala mutants incorporating labeled 1, non-cyclized (red) and cyclized (green) peptides. Asterisks indicate GSH addition products.