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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Pancreas. 2016 May-Jun;45(5):720–729. doi: 10.1097/MPA.0000000000000518

Figure 7.

Figure 7

A: MDA level of human pancreatic tissue in vitro. Human pancreatic tissue was cultured for 24 hours in media containing 0, 250 and 500 nM dh404 with or without H2O2 (200µM). The addition of H2O2 resulted in a significant rise in MDA level which was reduced in a dh404 concentration dependent manner.

B: Viability assay of human pancreatic cells. To test cytoprotective effects of dh404, viability assay in human pancreatic cells was performed using flow cytometry after H2O2 treatment. After dissociation of pancreatic cell, single-cell suspensions were stained with 7-AAD and TMRE. The percentage of TMRE- cells were calculated for apoptotic cells after the exclusion (gating out) of 7-AAD+ cells. The graph reveals a protective effect of dh404 (250nM) against oxidative stress. (* and * * indicate a statistically significant difference, p<0.05 and <0.001, respectively)