Fig 2. Identification of the M2-1 binding domain on P by GST pull-down.

(A) Schematic illustration of the full-length and truncated forms of GST-P used for M2-1 pulldowns in this study. The oligomerization domain of P is represented as a grey box, and numbers indicate amino acid positions. (B) GST-P proteins were purified on glutathione-Sepharose beads and incubated in the presence of M2-1. After extensive washing, the binding of M2-1 was determined by SDS-PAGE and Coomassie blue staining. For each deletion mutant, the ability to interact with M2-1 is summarized on the right in A.